Relationship between Production Traits and DNA Markers on the Z Chromosome in the 5th Generation of selection program of Silky Fowl.


MD. Mijanur Rahman1, 2, Yuki Nishiyama2, Ken-ichi Yamanaka1,2, Kajio Anann3, Yasuhiko Wada1,2


1 The United Graduate School of Agriculture, Kagoshima University, Kagoshima, 890-5880, Japan

2 Faculty of Agriculture, Saga University, Saga, 840-8502, Japan

3 Livestock Research Institute, Oita Prefectural Agriculture, Forestry and Fisheries Research Center, Bungo-Ohno 879-7111, Japan

Abstract

　The Silky fowl is a famous black-boned chicken breed; adult Silky fowl are characterized by low body weight and produces less number of eggs. Current research efforts have mainly focused on the improvement in egg production. Previous studies have indicated a relationship between egg production and DNA markers and a high genetic variation in Silky fowls in the Oita’s selection population. We have embarked on the improvement in the rate of egg production in this specific population. Some valuable markers related with egg production include the prolactin receptor and the growth hormone receptor, which were localized to the Z chromosome. In this study, five DNA markers on the Z chromosome were analyzed in the 5th generation of the Oita’s Silky fowl population. Genotyping of five DNA markers was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to study the relationship between DNA marker and production trait. The relationship was analyzed by linear mixed model using SAS Mixed procedure. Hatching group and genotype effects of five DNA markers were included as fixed effect in the model. Sire and dam effect was included as random effect. Genotypes and trait values were obtained for 202 female and 58 male individuals of the 5th generation. In the females, rs16773406 showed significance for egg production rate (p < 0.05), and for body weight at 150 days of age (p < 0.001). Markers rs15991083 showed significance for average egg weight (p < 0.05). In the males, embigin and rs15991083 showed significance for body weight at 150 days of age (p < 0.05), whereas rs16763148 and rs16773406 showed significance for body weight at 300 days of age (p < 0.05).

J. Warm Regional Society of Animal Science, Japan. 57 (2): 115-122. 2014.

烏骨鶏選抜集団第5世代におけるZ染色体上のDNAマーカーと生産形質との関連性


MD ミザヌル・ラハマン1,2、西山由紀2、山中賢一1,2、阿南加治男3、和田康彦1,2

1 鹿児島大学大学院連合農学研究科　鹿児島市 890-5880

2 佐賀大学農学部　佐賀市　 840-8502

3 大分県農林水産研究指導センター畜産研究部豚・鶏チーム　豊後大野市　879-7111

要約

烏骨鶏は有名な黒色の骨を有する鶏品種で、体重は小さく、産卵数も少なく、改良の必要性が指摘されている。以前の研究で、大分県の烏骨鶏の選抜集団において生産形質とDNAマーカーとの関連性が示されている。そこで、本研究ではプロラクチン受容体や成長ホルモン受容体が座乗しているZ染色体上のDNAマーカーについて検討した。大分県の烏骨鶏選抜集団の第5世代の、雌202羽、雄58羽について、Z染色体上の5つのDNAマーカーについてPCR-RFLPで遺伝子型判定を行い、SASのMixed Procedureを用いて、父鶏と母鶏を変量効果とした混合線形モデルで遺伝子型と生産形質との間の関連性について検討した。その結果、rs16773406では産卵率(p <0.05)と150日齢体重(p<0.001)で有意な関連性が認められた。また、rs159910838では平均卵重で有意な関連性が認められた(p<0.05)。雄ではエンビギンとrs15991083で150日齢体重との間で有意な関連性が認められ、300日齢体重についてはrs16763148とrs16773406で有意な関連性が認められた(p<0.05)。

日本暖地畜産学会報　57(2): 115-122, 2014

佐賀大学農学部応用生物科学科　動物資源開発学分野　和田研究室