Development of 50 gene-associated microsatellite markers using BAC clones and the construction of a linkage map of swine chromosome 4

N. Fujishima-Kanaya1), D. Toki1), K. Suzuki1), T. Sawazaki1), H. Hiraiwa1,2), M. Iida1), T. Hayashi2), H. Uenishi2), Y. Wada3), Y. Ito4), T. Awata2)

1) STAFF Institute, Tsukuba, Ibaraki, Japan
2) Genome Research Department, National Institute of Agrobiological Sciences, Tsukuba, Japan 
3) Faculty of Agriculture, Saga University, Saga, Japan.
4) Physiology and Genetic Regulation Departmenet, National Institute of Agrobiological Sciences, Tsukuba, Japan

ABSTRACT

The development of informative polymorphic markers is essential for QTL mapping. We developed 50 microsatellite markers from BAC clones containing genes that were predicted to map swine chromosome 4 (SSC4) according to comparative analysis between human and swine chromosomes, and constructed a linkage map that consisted of 37 markers including 24 markers closely linked to genes in BAC clones. Microsatellite markers were developed by direct-sequencing of BAC clones and our results demonstrated that this method was effective for developing microsatellite markers in specific regions on chromosomes. Effective development of microsatellite markers closely linked to genes can further accelerate the comparative studies of chromosomes between different species.

                                                Animal Genetics 34(2) 135-141. 2003.

佐賀大学農学部応用生物科学科　動物資源開発学分野　和田研究室