A linkgae map of 243 DNA markers in an intercross of Gottingen miniature and Meishan pigs

S. Mikawa, T. Akita, N. Hisamatsu, Y. Inage, Y. Ito, E. Kobayashi,
H. Kusumoto, T. Matsumoto, H. Mikami, M. Minezawa, M. Miyake,
S. Shimanuki, C. Sugiyama, Y. Uchida, Y. Wada, S. Yanai, H. Yasue

Dep. Animal Breeding and Genetics
National Institute of Animal Industry
2 Ikenodai, Kukisaki-machi, Inashiki-gun,
Ibaraki 305, Japan

The Second Research Division
Society for Techno-Innovation of Agriculture, Forestry and Fisheries,
446-1
Ippaizuka, Kamiyokoba, Tsukuba, 
Ibaraki 305, Japan

Summary

A resources family of pigs has been constructed by using a boar of
Gottingen miniature pig and two sows Meishan pig as parents.
In the construction of the family, two F1 males and 18 F1 females 
were intercrossed to generate 143 F2 offspring. The members of 
the family were genotyped using 243 genetic markers including 
26 markers developed in our laboratory in order to generate linkage 
map of markers for use in detecting quantitative trait loci (QTLs) 
in the family. The markers consisted of 237 microsatelites, five 
PRE-1 markers, and one RFLP marker. The linkage map was revealed to 
cover all 18 autosomes and the X chromosome; and the total length 
of the sex-averaged linkage map was calculated to be 2561.9 cM.
Four out of the 26 markers develped in our laboratory exended the
current linkage map at the termini of chromosomes 1p, 5p, 11p, and 
Xq. The linkage maps of all the chromosomes except for chromosome 1 
were found to be longer in females than in males. Concerning 
chromosome 1, the length of the linkage map showed no difference 
between females and males, which was attributed to low recombination
rates between markers localized in the centromeric region in females.
The average ratio of female-to-male recombination was calculated to 
be 1.55.

Animal Genetics 30, 407-417 (1999)